Abstract：Objective To investigate the expression of preferentially expressed antigen of melanoma(PRAME) in bone marrow mononuclear cells and its correlation with prognosis in children with acute leukemia(AL).Methods The levels of PRAME were measured by semiquantitative reverse transcription polymerase chain reaction and the results were analyzed in 95 children with AL.Results There were no significant differences in the positive rates of PRAME between ANLL patients and ALL patients(60.0% vs 56.9%,P>0.05).No PRAME expression was detected in patients with complete remission(CR) and normal control subjects.In ANLL patients,the positive rates of PRAME for M3,M2 and M4 subtypes were 75.0%,62.5% and 60.0%,respectively.The levels of PRAME expression in effective group were significantly lower than those in ineffective group(P<0.01).Eight patients with high expression of PRAME before chemotherapy were followed-up.After chemotherapy,all 8 patients achieved CR with negative PRAME expression.However,the recurrence occurred 6-12 months after CR.Among the 8 patients,the levels of PRAME expression increased again before clinical recurrence in 5 and in clinical relapse in 3.Conclusion PRAME is a marker gene for pediatric AL.Dynamic detection of PRAME expression can evaluate the development of disease and total load of leukemic cells.Therefore,it is important to guide chemotherapy,predict relapse,monitor minimal residual disease and prevent relapse in children with AL.
范小菊； 梁昌达； 钟小红； 谢淑佩. PRAME基因在小儿急性白血病患者中的表达及其与预后的相关性[J]. 南昌大学学报（医学版）, 2012, 52(03): 45-.
FAN Xiao-ju,LIANG Chang-da,ZHONG Xiao-hong,XIE Shu-pei. Preferentially Expressed Antigen of Melanoma Expression and Its Correlation with Prognosis in Children with Acute Leukemia. , 2012, 52(03): 45-.
2012, Vol. 52 
